Basic Information

Reference Number

0000225607

Issuing Organization

University of Ottawa

Owner Organization

University of Ottawa

Solicitation Type

ACAN - Advance Contract Award Notice (Formal)

Solicitation Number

2022-00607-ACAN

Title

SINGLE-CELL WESTERN SYSTEM

Source ID

PU.AC.ON.11752.C97953

Agreement Types

Broader Public Sector (BPS)

Canadian Free Trade Agreement (CFTA)

Details

Location

Canada, Ontario, Ottawa

Delivery Point

University of Ottawa

Purchase Type

Duration: TBD

Description

BACKGROUND:
Notice is hereby given by the University of Ottawa of the intent to enter a contract with Protein Simple (Bio-Techne) to procure:

A Milo single-cell western system,
Equipped with a two-color microarray scanner, and
A computer including the Scout image analysis software to treat single-cell western data.

The above items will be installed at Roger Guidon Hall (Faculty of Medicine) at the University of Ottawa and integrated to the Ottawa Hospital Research Institute StemCore technological platform.

PROCESS:
Suppliers who consider their equipment functional, successfully tested, readily available and fully compliant to the ACAN minimum requirements may submit in writing a statement of specifications to the contact person identified in
this Notice, on or before the closing date of this Notice. In the statement of specifications, the supplier must unequivocally demonstrate how their equipment, at minimum, equals, or exceeds the stated requirements.

If no other supplier submits a statement of specifications, on or before the closing date of this Notice, the competitive requirements of the University of Ottawa will be considered having been met.

Following notification to suppliers not successful in unequivocally demonstrating that their statement of specifications equals or exceeds the requirements set out in this Notice, the contract may then be awarded to the pre-identified
supplier.

Date of issue: June 7, 2022
Closing Date: June 22. 2022 at 3:00:00 P.M. Eastern Standard Time

INTENDED USE:
The above-described items constituting a single-cell western analysis platform represent a complete system that can be operated by the uOttawa research personnel.

The system consists of three main pieces of equipment:
i) Milo single cell western system:

Based on in-chip electrophoretic migration principle
Capturing/isolating single-cells between 7 to 25 m of diameter
Performing in-chip cell lysis
Enabling simultaneous analysis of 1000 single cells per run
Power requirement; 100-240 VAC input, 50/60 Hz, 2A/1A Maximum rated input current
HV circuit rating; 300 V, 2.5 A max

ii) Two-color microarray scanner, InnoScan 710, superior or equivalent:

Resolution of 3 - 40μm/pixel
Equipped with 532nm and 635nm lasers enabling the detection of Cy3, Alexa Fluor 546, Alexa Fluor 555, Cy5, Alexa Fluor 647 & Alexa Fluor 660 fluorophores.
Detection type: Real-time confocal with 2 photomultipliers (PMT). PMT gain adjustable from 0 to 100% (linear 1 to 100%).
Optical filter: 1 standard fluorescent filter by detection channel with real-time autofocus
- Manual focus: offset adjustment 300 μm range, 1 μm increment
Slide size: Compatible with all standard microscope slides: 25-26 x 75-76 mm2 / 1” x 3”; thickness: 0.9 - 1.2mm
Scanning area: Adjustable up to 22 x 74 mm2
Image format: TIFF (16-bit and 20-bit in dynamic extension mode)
Electrical: ~100-240 VAC, 1.2 A, 50-60 Hz

iii) Computer equipped with the Scout image analysis software:

Hardware (Apple or PC) with 16 GB RAM, line voltage: 100–240V AC, frequency: 50Hz to 60Hz, single phase.
64-bit versions of Windows 7 and 10 or Mac OS-X 10.11 (El Capitan), 10.12 (Sierra), 10.13 (High Sierra).
Equipped with the single-cell analysis Scout software v2.2.1beta (or higher).

Justification of Pre-Selected Supplier:

Supplier: Protein Simple (Bio-Techne)

Justification:

The single-cell western system must be based on the technology described by Hughes et al. (Nature Methods, 2014) using polyacrylamide gel chips with integrated cell-capturing micro wells, and able to achieve size-based protein separation on ~1,000 single-cells per biological sample. The system must include a high-resolution chip scanner (or equivalent technology) offering a multiplexing capacity of up to four markers per run (e.g. 532nm and 635nm lasers used to simultaneously detect 2 sets of markers with different molecular weights). The single-cell western system must enable measurement of protein targets irrespective of their location in or on a cell. It must also enable discrimination between isoforms, unspecific antigen binding (inaccurate molecular weight), and size shifts due to posttranslational and/or proteolytic processing. The cost of specialized consumables per run (excluding antibodies) must not exceed ~$400 CAD. The system must allow the use of standard commercial primary antibodies.
The single-cell western system must run on a user-friendly image analysis software enabling automated area under the curve calculations for immunodetection signal to quantify target abundance in each of the thousand single cells analyzed per run. The software must integrate the single-cell western data to portrait cell subpopulations across the heterogeneity of a sample. The software must be available with multiple licenses to enable stand-alone desktop analysis by the users.

Dates

Publication

2022/06/07 07:35:34 PM EDT

Bid Intent

Not Available

Question Acceptance Deadline

2022/06/15 12:00:00 AM EDT

Questions are submitted online

Yes

Closing Date

2022/06/22 03:00:00 PM EDT

Contact Information

Charles Gosselin

613-562-5800 ext. 1943

cgossel2@uottawa.ca

Buyer Preferences, Guidelines & Requirements

General Requirements

- Installation Required

- Insurance Required

- Training Required

- Warranty Information Required

Bid Submission Process

Bid Submission Type

Electronic Bid Submission

Pricing

No Pricing

Bid Documents List

Item Name	Description	Mandatory	Limited to 1 file
Statement of Compliance	Substantiation of Compliance with each Functional Minimum Requirement	Yes	No
No entries

© MERX - All rights reserved. No part of the information on this Web Site may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise without the prior permission of MERX and the Minister of the participating government department (if applicable). MERX, the Minister nor the Contracting Authority will assume responsibility or liability for the accuracy of the information contained in the publication.