Information de base

Numéro de référence

0000207195

Organisation émettrice

University of Ottawa

Organisation propriétaire

University of Ottawa

Type d’appel d’offres

DDP - Demande de proposition (Formel)

Numéro d’appel d’offres

40155-3A-RFP

Titre

Dual Output Laser System

Code d’identité d’origine

PU.AC.ON.11752.C97953

Types d’accord

Accord de libre-échange canadien (ALÉC)

Canada-Union Européenne: Accord économique et commercial Global (AECG)

Détails

Emplacement

Canada, Ontario, Ottawa

Point de livraison

University of Ottawa

Type d’achat

Durée: TBD

Description

Statement of Requirements

The University of Ottawa is pleased to invite qualified proponents to submit a proposal for a Dual Output Laser System that will
be part of of a 3D Acoustic Optical Deflector (AOD) Based Random Access Multi-Photon (RAMP) Microscope System that will
be used to collect data from custom defined positions at high temporal precision. The primary use of the system will be to monitor
synaptic signals and action potentials from subcellular neuronal compartments and large number of neuronal somas. In addition
to monitoring physiological signals, the system will also be used for stimulating neuronal elements through optogenetic methods
and uncaging. The system must be able to perfrom two-photon RAMP imaging and laser photo-activation system to execute
neuroscience experiments compatible with live cell imaging, voltage imaging and synchronized electrophysiology in brain slices
in vitro and in in vivo experimental designs. The system should be optimized for fast imaging from small custom defined Region
of Interest (ROIs) while simultaneously allowing experimental manipulations (i.e. uncaging).

General Description:

The objective of the research is to understand the physiological processes by which the brain translates incoming information
and code new memories. In order to achieve this, image neuronal activity at a very high speed is required. The microscope
system will be used to monitor neuronal voltage signals at high temporal and spatial resolution. Data obtained with the new
imaging system will expand research aiming to unveil new knowledge about memory formation and spatial navigation using novel
imaging and computational technologies that can be identified in future experiments as therapeutic targets for the treatment of
dementia.

The primary use of this system will be to study a) voltage signals in dendritic compartments and axonal terminals and b)
physiological responses from large number of neurons simultaneously. Imaging will be done in 3D custom defined locations which
could include various locations along the same dendritic tree or identified somas in from a larger volume. The system will be used
for high frequency recording of neural signals from spatially separated and distinct subcellular compartments and for the
synchronous stimulation of multiple synaptic inputs terminating on various dendritic regions at a kilohertz rate. The intended use
of the system will be to detect single action potentials in trains and subthreshold voltage changes in single trials, with sub
millisecond temporal resolution.

Mandatory Minimum Requirements for the Dual Output Laser System for AOD Multiphoton Microscope:

1. Dual Output Laser System
1.1 - The system has a dual output laser head.
1.2 - Minimum Tunable Output Wavelength range 700-1200nm.
1.3 - Fixed output wavelength range 1030 ± 15nm.
1.4 - Tunable Output : Pulse Duration FWHM (full width at half maximum) (100 fs ≤ t ≤ 400 fs).
1.5 - Tunable Output. Pulse Bandwidth FWHM (full width at half maximum) (bw ≤ 10nm @ 900nm).
1.6 - Tunable Output Power (> 2W @ 900nm).
1.7 - Tunable Output . Beam diameter 1.1 ± 1mm (@900nm).
1.8 - Tunable Output M² < 1.2.
1.9 - Tunable Output Noise < 0.5% RMS (root mean square).
1.10 - Tunaable Output Ellipticity 0.8-1.2.
1.11 - Fixed Output : Pulse Duration FWHM (full width at half maximum) (140 fs ≤ t ≤ 400 fs).
1.12 - Fixed Output : Pulse Bandwidth FWHM (full width at half maximum) (bw ≤ 10nm @ λc).
1.13 - Fixed Output Power (> 2W @ λc).
1.14 - Fixed Output Beam diameter 1.1 ± 1mm (@λc).
1.15 - Fixed Output M² < 1.2.
1.16 - Fixed Output Noise < 0.25% RMS (root mean square).

2. System Dimensions
2.1 - The systems must fit on an optical table within the available rectangular dimension of dimension 950mm W x 510mm H. (in the corner of an optical table). Please refer to Figure 1 – Optical Table Layout – Dual Output Laser System,below.
2.2 - Beam output height 110- 130 mm.
2.3 - The system must provide a stable fixation to the optical table (in the corner of an optical table with metric M6 taps @ 25mm grid starting at 12.5mm from the edge)

3. Warranty, Installation and Training
3.1 - Must include 1 year standard manufacturer’s warranty.
3.2 - Must include installation and on-site training.

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Dates

Publication

2021-08-25 14h45:31 HAE

Intention de soumissionner

Non disponible

Échéance de la réception des questions

2021-09-09 15h00:00 HAE

Questions sont soumises en ligne

Oui

Date de fermeture

2021-09-22 15h00:00 HAE

Information du contact

Charles Gosselin

613-562-5800 ext. 1943

cgossel2@uottawa.ca

Préférences, directives ou exigences de l’acheteur

Exigences générales

- Installation requise

- Assurances requises

- Formation requise

- Information sur la garantie obligatoire

Processus de soumission

Type de soumission

Soumission électronique

Fixation des prix

Dans un document attaché

Liste de documents de la soumission

Nom de l’item	Description	Obligatoire	Limité à un fichier
Attachment #1	Technical Qualitative Proposal	Oui	Non
Attachment #2	Financial Proposal	Oui	Non
Aucune entrée

Directives additionelles d’appel d’offres

No pricing should be included in Attachment #1 - Technical Qualitative Proposal
Please refer to RFP "Section 2.3 PROPOSAL SUBMITTED ONLY IN PRESCRIBED MANNER" for Proposal submition instructions.

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