Basic Information

Reference Number

0000241668

Issuing Organization

University of Ottawa

Owner Organization

University of Ottawa

Solicitation Type

RFP - Request for Proposal (Formal)

Solicitation Number

43581-2A-RFP

Title

Spinning Disc Confocal System

Source ID

PU.AC.ON.11752.C84282

Agreement Type

Broader Public Sector (BPS)

Details

Location

Canada, Ontario, Ottawa

Delivery Point

Ottawa

Purchase Type

One Time Only- Delivery Date:TBD

Description

The University requires spinning disk confocal microscope based on Nipkow disk equipped with micro lenses for fast imaging of intracellular dynamics in living cells in the brain sections.

The system must contain an upright microscope and will be used for

For imaging calcium signals propagation in the thin processes of developing cells
For imaging fast intracellular dynamics of organelles in distinct types of cells in the living brain sections

1. General Description of Instrument, System or Service

[Describe in a broad way what is to be achieved or delivered by the completion of the contract. Make sure to identify the intended use of the completed requirement. What is the output of the good or service. What are your expectations.]

The confocal microscope based on Nipkow disk equipped with micro lenses must have clearly separated light paths for excitation and emission, and even illumination. The spinning disk should allow for at least 10,000 rpm and 50 um pinhole size. The requested system is composed of an upright microscope equipped with a microscope controller, a motorized six-position fluorescent cube filter, and an epifluorescent module with appropriate filters and dichroics for imaging DAPI, GFP, TRITC and Cy5. The system should be equipped with four laser lines at 405, 488, 561 and 640 nm and a high sensitivity camera with 95% QE a sensor size of at least 18.8mm, 6.5x6.5um, 2048x2048 array size and low read noise. The camera should allow for 95fps at full array. A high numerical aperture (NA) 60x water-dipping objective (NA, 1.0) with apochromatic correction or better with corrections for Near Infra-Red observations with a 2.8-mm working distance (WD) and a 10x objective (NA, 0.45, WD 4.0 mm) are also needed. The system should be also equipped with a piezo z stage and a motorized xy stage with encoder as well as a workstation and software package to control the entire system and provide 2D/3D deconvolution options, object tracking, and 3D measurements.
The system will be used for tracking fast intracellular processes in different cell types in the brain sections

Dates

Publication

2023/02/17 05:11:38 PM EST

Bid Intent

Not Available

Question Acceptance Deadline

2023/03/03 12:00:00 PM EST

Questions are submitted online

Yes

Closing Date

2023/03/14 03:00:00 PM EDT

Contact Information

Roger Wills

613-562-5800 ext. 7113

rwills@uottawa.ca

Bid Submission Process

Bid Submission Type

Electronic Bid Submission

Pricing

In attached document

Bid Documents List

Item Name	Description	Mandatory	Limited to 1 file
Attachment # 1	Technical/Qualitative Proposal: 43581-2A-RFP-Spinning Disc Confocal System	Yes	No
Attachment # 2	Financial Proposal must include the following mandatory documents	No	No
No entries

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